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monoclonal rabbit anti alix antibody  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc monoclonal rabbit anti alix antibody
    Monoclonal Rabbit Anti Alix Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/alix/Alix+Rabbit+mAb/pm42887202-58-2-26
    Average 96 stars, based on 171 article reviews
    monoclonal rabbit anti alix antibody - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Polyacrylamide Gel Electrophoresis:

    Article Title: Sensor chip and methods thereof
    Article Snippet: Exosomes isolated by ultracentrifugation were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Thermo Scientific) and quantified using bicinchoninic acid assay (BCA assay, Thermo Scientific). .. Protein lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene fluoride membrane (PVDF, Invitrogen) and immunoblotted with antibodies against protein markers: HSP90 (Cell Signaling), HSP70 (BioLegend), Flotillin 1 (BD Biosciences), CD63 (Santa Cruz), ALIX (Cell Signaling), TSG101 (BD Biosciences), LAMP-1 (R&D Systems) and neuronal marker NCAM (R&D Systems). .. Following incubation with horseradish peroxidaseconjugated secondary antibody (Cell Signaling), enhanced chemiluminescence was used for immunodetection (Thermo Scientific).

    Membrane:

    Article Title: Sensor chip and methods thereof
    Article Snippet: Exosomes isolated by ultracentrifugation were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Thermo Scientific) and quantified using bicinchoninic acid assay (BCA assay, Thermo Scientific). .. Protein lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene fluoride membrane (PVDF, Invitrogen) and immunoblotted with antibodies against protein markers: HSP90 (Cell Signaling), HSP70 (BioLegend), Flotillin 1 (BD Biosciences), CD63 (Santa Cruz), ALIX (Cell Signaling), TSG101 (BD Biosciences), LAMP-1 (R&D Systems) and neuronal marker NCAM (R&D Systems). .. Following incubation with horseradish peroxidaseconjugated secondary antibody (Cell Signaling), enhanced chemiluminescence was used for immunodetection (Thermo Scientific).

    Marker:

    Article Title: Sensor chip and methods thereof
    Article Snippet: Exosomes isolated by ultracentrifugation were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease inhibitors (Thermo Scientific) and quantified using bicinchoninic acid assay (BCA assay, Thermo Scientific). .. Protein lysates were resolved by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), transferred onto polyvinylidene fluoride membrane (PVDF, Invitrogen) and immunoblotted with antibodies against protein markers: HSP90 (Cell Signaling), HSP70 (BioLegend), Flotillin 1 (BD Biosciences), CD63 (Santa Cruz), ALIX (Cell Signaling), TSG101 (BD Biosciences), LAMP-1 (R&D Systems) and neuronal marker NCAM (R&D Systems). .. Following incubation with horseradish peroxidaseconjugated secondary antibody (Cell Signaling), enhanced chemiluminescence was used for immunodetection (Thermo Scientific).

    other:


    Transmission Assay:

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation.
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Electron Microscopy:

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation.
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Transmission Electron Microscopy:

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation.
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Morphology:

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation.
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Western Blot:

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation.
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Expressing:

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.

    Article Title: Mesenchymal stem cells derived extracellular vesicles ameliorate ovarian aging through inhibiting LGALS3BP/NF-κB induced inflammation.
    Article Snippet: The particle concentration and size distribution were quantified by nanoparticle tracking analysis (NTA) (ZetaView, Particle Metrix, Germany). .. EVs identity was confirmed by transmission electron microscopy (TEM) for morphology, and Western blot was performed for surface markers of EVs (CD63 (25682-1-AP, Proteintech, China) and Alix (2171 S, CST, USA)) expression. .. LGALS3BP siRNAs (Sangon Biotech) were transfected into 70% confluent UCMSCs using LipoFiter 3.0 (Hanbio, HB-LF3-1000) following the manufacturer’s protocol.



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